Introduction: Peripheral blood is a large accessible source of adult stem cells for both basic research and clinical applications. Peripheral blood mononuclear cells (PBMCs) have been reported to contain distinct multipotent progenitor cell populations and has the potential to differentiate into different cells and tissues such as blood cells, endothelial cells, hepatocytes, cardiomyogenic cells, smooth muscle cells, or osteoblasts under appropriate conditions.
Objective : To develope an erythroid precursor model using CD34- peripheral blood mononuclear cells.
Results and Discussion : We managed to develop erythroid precursors using CD34 negative cells seperated by negative selection using magnatic beads from peripheral blood. The ability to develop erythroid precursors was tested using CD34 positive and CD34 negative cells. CD34 positive cells showed to a higher potential to produce erythroid precursors in the first seven days of culture with minimal erythroid differentition in the CD34 negative cells. On Day 14 many CD34 negative cells managed to differentiate into erythroid precursors with positive CD71 and Glycophorin A markers . This suggest that the CD34 negative cells have a capacity to differentiate into erythroid precursors and further investigations are needed to identify the nature of the invloved cells.