Examination of peripheral blood smear is one of the hematological examinations that made to observe the morphology of blood cells both erythrocytes, leukocytes and platelets. Peripheral blood smear make from spread of peripheral blood with anti-coagulant. Mangrove leaves (Avicennia alba) containing flavonoids and sulfates have anti-coagulant activity. This research is a preliminary study to assess the anti-coagulant activity of mangroves leaves (Avicennia alba) used to make peripheral blood smear. Avicennia alba leaves were collected from Teluk Ekas, Kecamatan Jerowaru, Kabupaten Lombok Timur, Nusa Tenggara Barat, Indonesia. Mangrove leaves are macerated with 96% ethanol (3x24h). The extract obtained was fractionated to obtain the n-hexane, chloroform, and residual fractions. The anticoagulant activity of ethanol extract and the three fractions were tested in vitro to 5 human blood samples by the method of Lee-White and Eustrek. Compared to heparin as a positive control. The shape of blood cells was analyzed descriptively. The results of this study were ethanol extract was positive contains metabolites secondary such as alkaloids, tannin, saponin, flavonoids, and terpenoid. The results of Lee-White method are ethanol extract (1 mg/mL) and three fractions with (0,5mg/mL) have anticoagulant activity. Meanwhile in Eustrek method ethanol extract and the three fractions showed blood cells has a good shape in peripheral blood smear.
Keywords: Avicennia alba leaves, anticoagulant, lee white and eustrek method, peripheral blood smear